Document Type : Original Article
Authors
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1 Packing and Packaging Materials Department, National Research Centre, Giza, Egypt 2 Advanced Materials and Nanotechnology Lab., Center of Excellence, National Research Centre, Giza, Egypt
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2 Advanced Materials and Nanotechnology Lab., Center of Excellence, National Research Centre, Giza, Egypt 3 Polymers and Pigments Department, National Research Centre, Giza, Egypt
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1 Packing and Packaging Materials Department, National Research Centre, Giza, Egypt
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2 Advanced Materials and Nanotechnology Lab., Center of Excellence, National Research Centre, Giza, Egypt 4 Applied Organic Chemistry Department, National Research Centre, Giza, Egypt
Abstract
In the context of diligent efforts directed to develop new polymeric matrices for enzyme immobilization, we developed carrageenan gel disks activated with bio-inspired polydopamine layer. The carrageenan gel disks were firstly treated with polycationic polymer (hyperbranched polyamidoamine) followed by a polydopamine layer. Such a layer can act as an active platform for the covalent immobilization of enzymes via Michael addition and Schiff base. The mechanical strength of the coated gel disks was obviously enhanced. β-galactosidase was successfully immobilized with a loading efficiency of about 52 %. Furthermore, the immobilized β-galactosidase exhibited enhanced activity at a broader range of pH and temperature. Additionally, the immobilized β-galactosidase preserved around 80 % of the initial activity during the eleven reusability cycles. Generally, activation of the gel disks with the bio-inspired polydopamine layer considered as a safe alternative to the glutaraldehyde due to its cytotoxic. Accordingly, the easily formed carrageenan gel disks coated with polydopamine could have potential as enzyme carrier in drug and food industries.
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